It is a direct sequel to Assassin's Creed II, with Ezio Auditore da Firenze returning as the main protagonist.
PlayStation 3 and Xbox 360 versions of the game are set to be released on November 16, 2010 in America and November 19, 2010 in Europe.
A Windows version will be released in Q1 2011.
It is the first game in the main series to feature a multiplayer mode.
Brotherhood will keep its sandbox style gameplay in an open world of Italy.
Like the previous installments, the gameplay's core mechanics are based on parkour movements, crowd-blending stealth, assassinations and melee fighting system.
The combat system will have a bunch of improvements and for the first time in the series, the game offers a multiplayer mode alongside 15-20 hours of singleplayer gameplay.
Assassin's Creed: Brotherhood is the first game in the main series to feature full multiplayer mode on all platforms.
The unique multiplayer mode was heralded by many at E3 2010 where it was showcased.
It received recognition from the media.
GameTrailers awarded it as the Best Multiplayer Game of the show.
The players are Templars in training at the Abstergo facility.
They use the animi (plural for animus) seen at the beginning of Assassin's Creed II to access memories of Assassins and then to acquire their skills using the "bleeding effect".
There will be various game modes and different maps, including the areas from the first and the second game like Florence, as well as new maps like Rome, Castel Gandolfo and Siena.
The gameplay in multiplayer mode is similar to the core gameplay of the series, as players are required to use their assassination and stealth skills.
Players earn points by completing each assassination without being detected and escaping after each kill.
Breaking the cover makes player visible to other gamers and makes them easier to assassinate.
The multiplayer mode also includes a leveling system, which allows players to unlock rewards by earning EXP points during the matches and gaining new levels.
Players can then unlock abilities, perks and streaks.
Abilities are active skills, which can be used again only after a cooldown time.
Perks are passive skills, which can be equipped before the match and they are active all the time.
Streaks are bonuses awarded for reaching certain number of successful or failed contracts.
The multiplayer beta was announced at E3 2010 and it will be exclusive for PlayStation 3 users.
It began on 4 October 2010, with early access for PlayStation Plus subscribers from 27 September, and ended on 18 October 2010.
Three maps have been confirmed for the beta: Rome, Castel Gandolfo and Siena.